Genotyping and primer details


Genotyping and primer details for 135D07 - At3g51260
Line ID 135D07
Locus At3g51260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19031074 of line 135D07 Chr3:19031074
   
Insertion allele amplicon 8474 - 01ex
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01ex: 5'-GTGACTTATCCTGCCTTCGTTTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19030710
   
Insertion allele amplicon 8474 - CK07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CK07: 5'-AGATCTATTGGAGCAAGAGCTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19031698
   
wt amplicon (over InsPos) 01ex - CK07
wt amplicon start Chr3:19030710
wt amplicon end Chr3:19031698
wt amplicon size 989 bp
Locus specific primer CK07: 5'-AGATCTATTGGAGCAAGAGCTCAG-3'
2nd locus specific primer 01ex: 5'-GTGACTTATCCTGCCTTCGTTTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11