Genotyping and primer details


Genotyping and primer details for 136D11 - At5g11330
Line ID 136D11
Locus At5g11330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3618449 of line 136D11 Chr5:3618449
   
Insertion allele amplicon 3144 - HP85
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer HP85: 5'-CTTATCCACCCAGAGGTTTCATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3618947
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11