Genotyping and primer details


Genotyping and primer details for 142B04 - At5g46340
Line ID 142B04
Locus At5g46340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18799508 of line 142B04 Chr5:18799508
   
Insertion allele amplicon 8474 - MP18
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MP18: 5'-AGACGGAAATATTGTCGAGTCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18800088
   
wt amplicon (over InsPos) 04cj - MP18
wt amplicon start Chr5:18799134
wt amplicon end Chr5:18800088
wt amplicon size 955 bp
Locus specific primer MP18: 5'-AGACGGAAATATTGTCGAGTCAGT-3'
2nd locus specific primer 04cj: 5'-TAGATGGAGATCAGTATCAAGCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11