Genotyping and primer details


Genotyping and primer details for 143E09 - At1g59870
Line ID 143E09
Locus At1g59870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22036446 of line 143E09 Chr1:22036446
   
Insertion allele amplicon 3144 - AJ04
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AJ04: 5'-TTTGCAAAGACACAATAGTTGGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22036004
   
Insertion allele amplicon 8474 - VL58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer VL58: 5'-TTGTTGAATCAGAAACACCAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22037019
   
wt amplicon (over InsPos) VL58 - AJ04
wt amplicon start Chr1:22036004
wt amplicon end Chr1:22037019
wt amplicon size 1016 bp
Locus specific primer AJ04: 5'-TTTGCAAAGACACAATAGTTGGAG-3'
2nd locus specific primer VL58: 5'-TTGTTGAATCAGAAACACCAGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11