Genotyping and primer details


Genotyping and primer details for 145C05 - At3g03200
Line ID 145C05
Locus At3g03200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:736458 of line 145C05 Chr3:736458
   
Insertion allele amplicon 8474 - MZ03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MZ03: 5'-TGACGCTTGTACTCCATTCAAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:735929
   
wt amplicon (over InsPos) 06ks - MZ03
wt amplicon start Chr3:735929
wt amplicon end Chr3:736899
wt amplicon size 971 bp
Locus specific primer MZ03: 5'-TGACGCTTGTACTCCATTCAAATA-3'
2nd locus specific primer 06ks: 5'-AACCACAATAATAGCGTTGGTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11