Genotyping and primer details


Genotyping and primer details for 145H10 - At1g21770
Line ID 145H10
Locus At1g21770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7652263 of line 145H10 Chr1:7652263
   
Insertion allele amplicon 8474 - FK28
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FK28: 5'-GAATTCAAATTCCAAATCCAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7652861
   
Insertion allele amplicon 3144 - KB40
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KB40: 5'-ATTAGTGTTTGTTTGTTGCTGTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7651691
   
wt amplicon (over InsPos) 44rd - KB40
wt amplicon start Chr1:7651691
wt amplicon end Chr1:7652563
wt amplicon size 873 bp
Locus specific primer KB40: 5'-ATTAGTGTTTGTTTGTTGCTGTCG-3'
2nd locus specific primer 44rd: 5'-GATCGGACTTTTTGAAAGAACAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11