Genotyping and primer details


Genotyping and primer details for 148C12 - At4g28490
Line ID 148C12
Locus At4g28490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14079427 of line 148C12 Chr4:14079427
   
Insertion allele amplicon 8474 - IS42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS42: 5'-GAACTCAAGCTTTTCAACAACAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14078896
   
wt amplicon (over InsPos) 14gl - IS42
wt amplicon start Chr4:14078896
wt amplicon end Chr4:14080011
wt amplicon size 1116 bp
Locus specific primer IS42: 5'-GAACTCAAGCTTTTCAACAACAGA-3'
2nd locus specific primer 14gl: 5'-TTCTCCACCTCTAAGCTCAACTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11