Genotyping and primer details


Genotyping and primer details for 148H03 - At4g27830
Line ID 148H03
Locus At4g27830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13862985 of line 148H03 Chr4:13862985
   
Insertion allele amplicon 8474 - 58wg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 58wg: 5'-CCATCGGCATACCTGTTTTTATATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13862195
   
Insertion allele amplicon 3144 - VL15
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer VL15: 5'-CATTGGTTCTTATGACCAAGGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13863247
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11