Genotyping and primer details

Genotyping and primer details for 180A11 - At3g07390
Line ID 180A11
Locus At3g07390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2365434 of line 180A11 Chr3:2365434
Insertion allele amplicon 8474 - 42ms
Locus specific primer 42ms: 5'-CTTAGTCCCCGTAGGGTTAATAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2365784
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 14:50