Genotyping and primer details


Genotyping and primer details for 180D12 - At2g37340
Line ID 180D12
Locus At2g37340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15670891 of line 180D12 Chr2:15670891
   
Insertion allele amplicon 8474 - CK03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CK03: 5'-GCCATCAAAGTTAAGGAGACTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15670362
   
wt amplicon (over InsPos) 01gy - CK03
wt amplicon start Chr2:15670362
wt amplicon end Chr2:15671320
wt amplicon size 959 bp
Locus specific primer CK03: 5'-GCCATCAAAGTTAAGGAGACTCAC-3'
2nd locus specific primer 01gy: 5'-GTTGTTTGGCTCTCTATTTTCAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11