Genotyping and primer details


Genotyping and primer details for 193B03 - At4g01720
Line ID 193B03
Locus At4g01720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:745726 of line 193B03 Chr4:745726
   
Insertion allele amplicon 8474 - IS13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS13: 5'-AAAGCTGCACGTATTTATTCGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:746181
   
wt amplicon (over InsPos) 08cm - IS13
wt amplicon start Chr4:745471
wt amplicon end Chr4:746181
wt amplicon size 711 bp
Locus specific primer IS13: 5'-AAAGCTGCACGTATTTATTCGTCT-3'
2nd locus specific primer 08cm: 5'-TGTCAACAAAATAATCGGATGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11