Genotyping and primer details


Genotyping and primer details for 203G10 - T5P19
Line ID 203G10
Locus T5P19 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20909730 of line 203G10 Chr3:20909730
   
Insertion allele amplicon 3144 - CT42
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CT42: 5'-TAGGACTCTTGTGATCTTCGGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20909345
   
wt amplicon (over InsPos) IS14 - CT42
wt amplicon start Chr3:20909345
wt amplicon end Chr3:20910098
wt amplicon size 754 bp
Locus specific primer CT42: 5'-TAGGACTCTTGTGATCTTCGGAAT-3'
2nd locus specific primer IS14: 5'-AAGAAAGTAAGTTTCGCCCTTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11