Genotyping and primer details

Genotyping and primer details for 220B01 - At2g40950
Line ID 220B01
Locus At2g40950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17089107 of line 220B01 Chr2:17089107
Insertion allele amplicon 8474 - NE40
Locus specific primer NE40: 5'-AGACTCATGAGAAACCGTGAAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17089627
wt amplicon (over InsPos) 93zj - NE40
wt amplicon start Chr2:17088058
wt amplicon end Chr2:17089627
wt amplicon size 1570 bp
Locus specific primer NE40: 5'-AGACTCATGAGAAACCGTGAAAGT-3'
2nd locus specific primer 93zj: 5'-AGGTTAAAATCTGATCCTGGGAGT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11