Genotyping and primer details


Genotyping and primer details for 239B09 - At1g01720
Line ID 239B09
Locus At1g01720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:269330 of line 239B09 Chr1:269330
   
Insertion allele amplicon 8474 - MZ12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MZ12: 5'-TTAAATTGATTGCGGCACATATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:269807
   
wt amplicon (over InsPos) 07cu - MZ12
wt amplicon start Chr1:268717
wt amplicon end Chr1:269807
wt amplicon size 1091 bp
Locus specific primer MZ12: 5'-TTAAATTGATTGCGGCACATATAA-3'
2nd locus specific primer 07cu: 5'-GTTTAGCCTTGTATGGTGAGAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11