Genotyping and primer details


Genotyping and primer details for 245F03 - At3g19760
Line ID 245F03
Locus At3g19760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6866205 of line 245F03 Chr3:6866205
   
Insertion allele amplicon 8474 - BA30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BA30: 5'-ACTCAACTCTAACTCCGCATTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6865609
   
Insertion allele amplicon 8474 - BA31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BA31: 5'-AATTTCATCAACTTGCTTTGCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6866635
   
wt amplicon (over InsPos) BA31 - BA30
wt amplicon start Chr3:6865609
wt amplicon end Chr3:6866635
wt amplicon size 1027 bp
Locus specific primer BA30: 5'-ACTCAACTCTAACTCCGCATTTGT-3'
2nd locus specific primer BA31: 5'-AATTTCATCAACTTGCTTTGCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11