Genotyping and primer details


Genotyping and primer details for 248D08 - At2g17370
Line ID 248D08
Locus At2g17370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7550173 of line 248D08 Chr2:7550173
   
Insertion allele amplicon 8474 - PP08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PP08: 5'-GCATTGAAGGTTTGAATTTTTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7549553
   
wt amplicon (over InsPos) 11bj - PP08
wt amplicon start Chr2:7549553
wt amplicon end Chr2:7550523
wt amplicon size 971 bp
Locus specific primer PP08: 5'-GCATTGAAGGTTTGAATTTTTGAT-3'
2nd locus specific primer 11bj: 5'-TTTTACAATCTCCGAGTTTCGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11