Genotyping and primer details


Genotyping and primer details for 260F08 - At5g35410
Line ID 260F08
Locus At5g35410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:13636868 of line 260F08 Chr5:13636868
   
Insertion allele amplicon 8474 - JK10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JK10: 5'-ACCGACATTCTTTTATTCGAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:13637162
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 02.09.2016 15:25