Genotyping and primer details


Genotyping and primer details for 265A07 - At2g27880
Line ID 265A07
Locus At2g27880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11872661 of line 265A07 Chr2:11872661
   
Insertion allele amplicon 8474 - FQ19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FQ19: 5'-AAAGACCTCCCAACAGAGACATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11872998
   
Insertion allele amplicon 8474 - PH29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH29: 5'-CGTTTGTGTTCATCTGAAGTTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11872195
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11