Genotyping and primer details


Genotyping and primer details for 270C03 - At3g48890
Line ID 270C03
Locus At3g48890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18130508 of line 270C03 Chr3:18130508
   
Insertion allele amplicon 3144 - 04am
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 04am: 5'-TCTCAGAGCAGGTATTTCTTGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18129966
   
Insertion allele amplicon 8474 - UF24
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer UF24: 5'-CTGCATAAAAAGAACAGATGGTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18130959
   
wt amplicon (over InsPos) UF24 - 04am
wt amplicon start Chr3:18129966
wt amplicon end Chr3:18130959
wt amplicon size 994 bp
Locus specific primer 04am: 5'-TCTCAGAGCAGGTATTTCTTGTGA-3'
2nd locus specific primer UF24: 5'-CTGCATAAAAAGAACAGATGGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11