Genotyping and primer details


Genotyping and primer details for 279D02 - At1g69570
Line ID 279D02
Locus At1g69570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26162827 of line 279D02 Chr1:26162827
   
Insertion allele amplicon 8474 - MZ20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MZ20: 5'-CTGACTGATGCTTACCAGTCTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26162289
   
wt amplicon (over InsPos) 18dk - MZ20
wt amplicon start Chr1:26162289
wt amplicon end Chr1:26163405
wt amplicon size 1117 bp
Locus specific primer MZ20: 5'-CTGACTGATGCTTACCAGTCTCTG-3'
2nd locus specific primer 18dk: 5'-TTTCTCTCTTCCTCTCTCCCTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11