Genotyping and primer details


Genotyping and primer details for 283D09 - At4g28520
Line ID 283D09
Locus At4g28520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14087898 of line 283D09 Chr4:14087898
   
Insertion allele amplicon 3144 - 10au
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 10au: 5'-GCTGTTCTCCTGAGTTGTAGATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14088384
   
Insertion allele amplicon 8474 - KR02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KR02: 5'-CTCACAGAGTGACGTGTAAAAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14087448
   
wt amplicon (over InsPos) 10au - KR02
wt amplicon start Chr4:14087448
wt amplicon end Chr4:14088384
wt amplicon size 937 bp
Locus specific primer KR02: 5'-CTCACAGAGTGACGTGTAAAAAGC-3'
2nd locus specific primer 10au: 5'-GCTGTTCTCCTGAGTTGTAGATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11