Genotyping and primer details


Genotyping and primer details for 290D11 - At4g18240
Line ID 290D11
Locus At4g18240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10085882 of line 290D11 Chr4:10085882
   
Insertion allele amplicon 3144 - 50xd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 50xd: 5'-GTTTGATTTATTTCACTGCAGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10084861
   
Insertion allele amplicon 8474 - SS02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer SS02: 5'-GTTGTTCAATACCTTCAAATTCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10086280
   
wt amplicon (over InsPos) CT36 - SS02
wt amplicon start Chr4:10085514
wt amplicon end Chr4:10086280
wt amplicon size 767 bp
Locus specific primer SS02: 5'-GTTGTTCAATACCTTCAAATTCCC-3'
2nd locus specific primer CT36: 5'-GATCACAAAGCCAATATAAACCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11