Genotyping and primer details


Genotyping and primer details for 293B08 - At2g28800
Line ID 293B08
Locus At2g28800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12357197 of line 293B08 Chr2:12357197
   
Insertion allele amplicon 8474 - PH53
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH53: 5'-TGATGATCCTGCACAGAAGAATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12357554
   
wt amplicon (over InsPos) 13rd - PH53
wt amplicon start Chr2:12356636
wt amplicon end Chr2:12357554
wt amplicon size 919 bp
Locus specific primer PH53: 5'-TGATGATCCTGCACAGAAGAATAC-3'
2nd locus specific primer 13rd: 5'-TCTTCCTTACTTGCAATTTCGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11