Genotyping and primer details


Genotyping and primer details for 297D04 - At1g51190
Line ID 297D04
Locus At1g51190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18979579 of line 297D04 Chr1:18979579
   
Insertion allele amplicon 8474 - 05hd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05hd: 5'-TAAAGGCTGATGATGATGAAGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18979909
   
wt amplicon (over InsPos) 05ig - 05hd
wt amplicon start Chr1:18979149
wt amplicon end Chr1:18979909
wt amplicon size 761 bp
Locus specific primer 05hd: 5'-TAAAGGCTGATGATGATGAAGATG-3'
2nd locus specific primer 05ig: 5'-AATTAAAGAACCTCGCGTAAACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11