Genotyping and primer details


Genotyping and primer details for 314F05 - At1g74430
Line ID 314F05
Locus At1g74430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27976413 of line 314F05 Chr1:27976413
   
Insertion allele amplicon 8474 - RS08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RS08: 5'-GATTCAAAGAAGGAACAGGTCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27977121
   
wt amplicon (over InsPos) 43dy - RS08
wt amplicon start Chr1:27976064
wt amplicon end Chr1:27977121
wt amplicon size 1058 bp
Locus specific primer RS08: 5'-GATTCAAAGAAGGAACAGGTCAAG-3'
2nd locus specific primer 43dy: 5'-AGTGTTTCCCTGGTTCCTATTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11