Genotyping and primer details


Genotyping and primer details for 316D04 - At3g25530
Line ID 316D04
Locus At3g25530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9272819 of line 316D04 Chr3:9272819
   
Insertion allele amplicon 8474 - MJ61
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MJ61: 5'-AAGAGCTAGCCTCATGTCTTTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9272193
   
wt amplicon (over InsPos) 19hf - MJ61
wt amplicon start Chr3:9272193
wt amplicon end Chr3:9273117
wt amplicon size 925 bp
Locus specific primer MJ61: 5'-AAGAGCTAGCCTCATGTCTTTCTG-3'
2nd locus specific primer 19hf: 5'-AGCATTGGTGATGAATGATATGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11