Genotyping and primer details


Genotyping and primer details for 320D03 - At2g40000
Line ID 320D03
Locus At2g40000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16701100 of line 320D03 Chr2:16701100
   
Insertion allele amplicon 8474 - IS61
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS61: 5'-TCAAATATGGATCGTCAAATGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16700534
   
wt amplicon (over InsPos) 32da - IS61
wt amplicon start Chr2:16700534
wt amplicon end Chr2:16701560
wt amplicon size 1027 bp
Locus specific primer IS61: 5'-TCAAATATGGATCGTCAAATGTTC-3'
2nd locus specific primer 32da: 5'-GAAGAAGAAGGTATATCAGCGGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11