Genotyping and primer details


Genotyping and primer details for 321C02 - At1g68590
Line ID 321C02
Locus At1g68590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25758247 of line 321C02 Chr1:25758247
   
Insertion allele amplicon 8474 - DE23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DE23: 5'-GAGATTGATGATATCAGTGGCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25757629
   
wt amplicon (over InsPos) 30go - DE23
wt amplicon start Chr1:25757629
wt amplicon end Chr1:25758819
wt amplicon size 1191 bp
Locus specific primer DE23: 5'-GAGATTGATGATATCAGTGGCTTG-3'
2nd locus specific primer 30go: 5'-CAGTGCTACTTCAAGTCCAAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11