Genotyping and primer details


Genotyping and primer details for 324D11 - At3g56400
Line ID 324D11
Locus At3g56400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20909793 of line 324D11 Chr3:20909793
   
Insertion allele amplicon 3144 - 89wr
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 89wr: 5'-AAAAAGTGCCCATCATTACAAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20910548
   
Insertion allele amplicon 8474 - IS32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS32: 5'-TAGGACTCTTGTGATCTTCGGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20909345
   
wt amplicon (over InsPos) 41lm - IS32
wt amplicon start Chr3:20909345
wt amplicon end Chr3:20910098
wt amplicon size 754 bp
Locus specific primer IS32: 5'-TAGGACTCTTGTGATCTTCGGAAT-3'
2nd locus specific primer 41lm: 5'-AAGAAAGTAAGTTTCGCCCTTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11