Genotyping and primer details


Genotyping and primer details for 328G03 - At4g31800
Line ID 328G03
Locus At4g31800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15383601 of line 328G03 Chr4:15383601
   
Insertion allele amplicon 8474 - IS33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS33: 5'-CAAATTGAGACTACGCACCAACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15383235
   
wt amplicon (over InsPos) 24km - IS33
wt amplicon start Chr4:15383235
wt amplicon end Chr4:15384032
wt amplicon size 798 bp
Locus specific primer IS33: 5'-CAAATTGAGACTACGCACCAACTA-3'
2nd locus specific primer 24km: 5'-TGAGTCCAATAGGAAAGCCTAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11