Genotyping and primer details


Genotyping and primer details for 355A07 - At1g12600
Line ID 355A07
Locus At1g12600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4287846 of line 355A07 Chr1:4287846
   
Insertion allele amplicon 8474 - MP49
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MP49: 5'-ACAGGGTATTTTCTTCTCAACCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4287507
   
wt amplicon (over InsPos) 06dw - MP49
wt amplicon start Chr1:4287507
wt amplicon end Chr1:4288134
wt amplicon size 628 bp
Locus specific primer MP49: 5'-ACAGGGTATTTTCTTCTCAACCCT-3'
2nd locus specific primer 06dw: 5'-CATTGCCTTGATTTACATGTATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11