Genotyping and primer details


Genotyping and primer details for 368H02 - At1g17880
Line ID 368H02
Locus At1g17880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6152583 of line 368H02 Chr1:6152583
   
Insertion allele amplicon 8474 - MJ37
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MJ37: 5'-TTAATGTAAACGTGTTGCTTCCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6152907
   
wt amplicon (over InsPos) 07bj - MJ37
wt amplicon start Chr1:6152030
wt amplicon end Chr1:6152907
wt amplicon size 878 bp
Locus specific primer MJ37: 5'-TTAATGTAAACGTGTTGCTTCCTG-3'
2nd locus specific primer 07bj: 5'-TGAACATCTCAAAGAATGTCTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11