Genotyping and primer details


Genotyping and primer details for 373H03 - At5g62575
Line ID 373H03
Locus At5g62575 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25118430 of line 373H03 Chr5:25118430
   
Insertion allele amplicon 8474 - MJ28
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MJ28: 5'-GTTGTTGATGCTTATGCTGTTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25117968
   
Insertion allele amplicon 8474 - MJ29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MJ29: 5'-ATCCTTTCGAAACAATTTCATGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25118936
   
wt amplicon (over InsPos) MJ29 - MJ28
wt amplicon start Chr5:25117968
wt amplicon end Chr5:25118936
wt amplicon size 969 bp
Locus specific primer MJ28: 5'-GTTGTTGATGCTTATGCTGTTGTT-3'
2nd locus specific primer MJ29: 5'-ATCCTTTCGAAACAATTTCATGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11