Genotyping and primer details


Genotyping and primer details for 379C07 - At1g17040
Line ID 379C07
Locus At1g17040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5825525 of line 379C07 Chr1:5825525
   
Insertion allele amplicon 8474 - XD03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer XD03: 5'-TTCCAGATGTAGATGGCTTATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5826101
   
wt amplicon (over InsPos) 11rd - XD03
wt amplicon start Chr1:5825198
wt amplicon end Chr1:5826101
wt amplicon size 904 bp
Locus specific primer XD03: 5'-TTCCAGATGTAGATGGCTTATCAA-3'
2nd locus specific primer 11rd: 5'-GACTTTCTCACTTTGTTTTTGGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11