Genotyping and primer details


Genotyping and primer details for 381F11 - At3g15950
Line ID 381F11
Locus At3g15950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5400011 of line 381F11 Chr3:5400011
   
Insertion allele amplicon 8474 - JE25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JE25: 5'-CCGATTCATCTAGTTATCAAGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5399406
   
Insertion allele amplicon 8474 - JL28
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL28: 5'-TGTAACAAAACATGGAAACAGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5399518
   
wt amplicon (over InsPos) 08fw - JL28
wt amplicon start Chr3:5399518
wt amplicon end Chr3:5400372
wt amplicon size 855 bp
Locus specific primer JL28: 5'-TGTAACAAAACATGGAAACAGAGC-3'
2nd locus specific primer 08fw: 5'-CAATTGAACGTGAGTTTGAAGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11