Genotyping and primer details


Genotyping and primer details for 387F09 - At3g55320
Line ID 387F09
Locus At3g55320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20511364 of line 387F09 Chr3:20511364
   
Insertion allele amplicon 8474 - KP68
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KP68: 5'-CTTCCGACACTAGTTTATTGCCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20511670
   
wt amplicon (over InsPos) 14hf - KP68
wt amplicon start Chr2:16481867
wt amplicon end Chr3:20511670
wt amplicon size 767 bp
Locus specific primer KP68: 5'-CTTCCGACACTAGTTTATTGCCTC-3'
2nd locus specific primer 14hf: 5'-TTTTCCAGAACCATTTCTACCAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11