Genotyping and primer details


Genotyping and primer details for 402B01 - At1g68650
Line ID 402B01
Locus At1g68650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25775763 of line 402B01 Chr1:25775763
   
Insertion allele amplicon 8474 - RK76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK76: 5'-AAAACATATACCGAAGCTGAGGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25776277
   
wt amplicon (over InsPos) 06dn - RK76
wt amplicon start Chr1:25775451
wt amplicon end Chr1:25776277
wt amplicon size 827 bp
Locus specific primer RK76: 5'-AAAACATATACCGAAGCTGAGGAG-3'
2nd locus specific primer 06dn: 5'-ATGTTGGTTCCTTTGTACCTTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11