Genotyping and primer details


Genotyping and primer details for 406G11 - At1g17460
Line ID 406G11
Locus At1g17460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6000845 of line 406G11 Chr1:6000845
   
Insertion allele amplicon 8474 - NC63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NC63: 5'-TATTCAATCCAGGCTTCTTTCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6000275
   
Insertion allele amplicon 8474 - NC64
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NC64: 5'-TAGCTCATATGATAAGGAATGGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6001384
   
wt amplicon (over InsPos) NC64 - NC63
wt amplicon start Chr1:6000275
wt amplicon end Chr1:6001384
wt amplicon size 1110 bp
Locus specific primer NC63: 5'-TATTCAATCCAGGCTTCTTTCAGT-3'
2nd locus specific primer NC64: 5'-TAGCTCATATGATAAGGAATGGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11