Genotyping and primer details


Genotyping and primer details for 406H11 - At2g30620
Line ID 406H11
Locus At2g30620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13045241 of line 406H11 Chr2:13045241
   
Insertion allele amplicon 8474 - JG78
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JG78: 5'-AGAAGCTTTAGCTGGTCTCTCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13046126
   
wt amplicon (over InsPos) CB33 - JG78
wt amplicon start Chr2:13045065
wt amplicon end Chr2:13046126
wt amplicon size 1062 bp
Locus specific primer JG78: 5'-AGAAGCTTTAGCTGGTCTCTCCTT-3'
2nd locus specific primer CB33: 5'-AAGCACCTATCCACATCCATTAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11