Genotyping and primer details


Genotyping and primer details for 407D08 - At1g54870
Line ID 407D08
Locus At1g54870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20459482 of line 407D08 Chr1:20459482
   
Insertion allele amplicon 8474 - JG76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JG76: 5'-CTTCCTTCCTTCATATGCTTCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20459963
   
Insertion allele amplicon 8474 - JG77
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JG77: 5'-GTTTACCAACACGTGTATCCTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20458897
   
wt amplicon (over InsPos) JG77 - JG76
wt amplicon start Chr1:20458897
wt amplicon end Chr1:20459963
wt amplicon size 1067 bp
Locus specific primer JG76: 5'-CTTCCTTCCTTCATATGCTTCAAC-3'
2nd locus specific primer JG77: 5'-GTTTACCAACACGTGTATCCTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11