Genotyping and primer details


Genotyping and primer details for 413B10 - At3g53810
Line ID 413B10
Locus At3g53810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19934898 of line 413B10 Chr3:19934898
   
Insertion allele amplicon 8474 - EQ55
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ55: 5'-CTCATTGACCATTGACCACTAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19935542
   
Insertion allele amplicon 8474 - KF65
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KF65: 5'-ATCAAAGGCATACCGATTTTGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19934297
   
wt amplicon (over InsPos) KF65 - EQ55
wt amplicon start Chr3:19934297
wt amplicon end Chr3:19935542
wt amplicon size 1246 bp
Locus specific primer EQ55: 5'-CTCATTGACCATTGACCACTAGAA-3'
2nd locus specific primer KF65: 5'-ATCAAAGGCATACCGATTTTGTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11