Genotyping and primer details


Genotyping and primer details for 432C09 - At3g50750
Line ID 432C09
Locus At3g50750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18862139 of line 432C09 Chr3:18862139
   
Insertion allele amplicon 8474 - 11bd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11bd: 5'-GGGAGTGGTTACTCTCACTTTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18861933
   
Insertion allele amplicon 8474 - 50wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 50wp: 5'-AAAACCAGTCAACCAAAACGTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18861421
   
Insertion allele amplicon 8474 - AY51
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AY51: 5'-AAGTCCTCAAAGCTCTTTGTCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18862890
   
wt amplicon (over InsPos) 11bd - AY51
wt amplicon start Chr3:18861933
wt amplicon end Chr3:18862890
wt amplicon size 958 bp
Locus specific primer AY51: 5'-AAGTCCTCAAAGCTCTTTGTCTTG-3'
2nd locus specific primer 11bd: 5'-GGGAGTGGTTACTCTCACTTTCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11