Genotyping and primer details


Genotyping and primer details for 455A02 - At2g26330
Line ID 455A02
Locus At2g26330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11209851 of line 455A02 Chr2:11209851
   
Insertion allele amplicon 8474 - EQ74
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ74: 5'-CTTACCATGAAGAAGATCCCAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11209250
   
Insertion allele amplicon 8474 - KU46
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU46: 5'-TATCCCTTCCAAGCTGTTAAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11210432
   
wt amplicon (over InsPos) KU46 - EQ74
wt amplicon start Chr2:11209250
wt amplicon end Chr2:11210432
wt amplicon size 1183 bp
Locus specific primer EQ74: 5'-CTTACCATGAAGAAGATCCCAGAG-3'
2nd locus specific primer KU46: 5'-TATCCCTTCCAAGCTGTTAAGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11