Genotyping and primer details


Genotyping and primer details for 476A11 - At5g27420
Line ID 476A11
Locus At5g27420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9685031 of line 476A11 Chr5:9685031
   
Insertion allele amplicon 8474 - AS40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AS40: 5'-AGTACAAATTTTTGTTTTGGTCCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9685688
   
wt amplicon (over InsPos) 12li - AS40
wt amplicon start Chr5:9684457
wt amplicon end Chr5:9685688
wt amplicon size 1232 bp
Locus specific primer AS40: 5'-AGTACAAATTTTTGTTTTGGTCCG-3'
2nd locus specific primer 12li: 5'-GACACAGAAGATTGGTAAAGGAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11