Genotyping and primer details


Genotyping and primer details for 493A10 - At2g02810
Line ID 493A10
Locus At2g02810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:803079 of line 493A10 Chr2:803079
   
Insertion allele amplicon 8474 - MP30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MP30: 5'-ACGGAATAAGATACACTTTCCCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:802518
   
wt amplicon (over InsPos) 08da - MP30
wt amplicon start Chr2:802518
wt amplicon end Chr2:803513
wt amplicon size 996 bp
Locus specific primer MP30: 5'-ACGGAATAAGATACACTTTCCCTG-3'
2nd locus specific primer 08da: 5'-GTTTACACCAACCCAAATTTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11