Genotyping and primer details


Genotyping and primer details for 493E02 - At4g27060
Line ID 493E02
Locus At4g27060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13582595 of line 493E02 Chr4:13582595
   
Insertion allele amplicon 8474 - BD81
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BD81: 5'-CCAGACTCTGCTTCTAAAGGAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13583159
   
wt amplicon (over InsPos) 61dy - BD81
wt amplicon start Chr4:13582219
wt amplicon end Chr4:13583159
wt amplicon size 941 bp
Locus specific primer BD81: 5'-CCAGACTCTGCTTCTAAAGGAAAA-3'
2nd locus specific primer 61dy: 5'-GCTTCAAGTGTAGCTTCATCCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11