Genotyping and primer details


Genotyping and primer details for 527D12 - At5g55120
Line ID 527D12
Locus At5g55120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22369822 of line 527D12 Chr5:22369822
   
Insertion allele amplicon 3144 - AR09
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AR09: 5'-GTAATCACGGCTATTTACGGGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22369252
   
Insertion allele amplicon 8474 - KF03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KF03: 5'-TAGCTTAGGCAAACATCAACAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22370175
   
wt amplicon (over InsPos) AJ18 - AR09
wt amplicon start Chr5:22369252
wt amplicon end Chr5:22370175
wt amplicon size 924 bp
Locus specific primer AR09: 5'-GTAATCACGGCTATTTACGGGATA-3'
2nd locus specific primer AJ18: 5'-TAGCTTAGGCAAACATCAACAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11