Genotyping and primer details


Genotyping and primer details for 550D06 - At1g15960
Line ID 550D06
Locus At1g15960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5483141 of line 550D06 Chr1:5483141
   
Insertion allele amplicon 8474 - RV01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RV01: 5'-TTGAGAAGTGGGACTAAAGCAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5482661
   
Insertion allele amplicon 8474 - RV02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RV02: 5'-CAGACCCAAAAGAAGTCCTCTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5483687
   
wt amplicon (over InsPos) RV01 - RV02
wt amplicon start Chr1:5482661
wt amplicon end Chr1:5483687
wt amplicon size 1026 bp
Locus specific primer RV02: 5'-CAGACCCAAAAGAAGTCCTCTATG-3'
2nd locus specific primer RV01: 5'-TTGAGAAGTGGGACTAAAGCAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11