Genotyping and primer details


Genotyping and primer details for 567F10 - At1g20980
Line ID 567F10
Locus At1g20980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7326247 of line 567F10 Chr1:7326247
   
Insertion allele amplicon 8474 - NJ37
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NJ37: 5'-CCAATATGGATCTTATGGCTTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7325784
   
wt amplicon (over InsPos) 04qy - NJ37
wt amplicon start Chr1:7325784
wt amplicon end Chr1:7326699
wt amplicon size 916 bp
Locus specific primer NJ37: 5'-CCAATATGGATCTTATGGCTTTGT-3'
2nd locus specific primer 04qy: 5'-AGTCAGAGCCAGAAGAAGCATAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11