Genotyping and primer details


Genotyping and primer details for 570H02 - At4g18020
Line ID 570H02
Locus At4g18020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10006815 of line 570H02 Chr4:10006815
   
Insertion allele amplicon 8474 - NA25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NA25: 5'-TTGCCTTTCTCCTTCATTAGTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10007114
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 31.05.2018 12:55